솔방울 에틸아세테이트 분획물의 항산화 및 산화적 DNA 손상 억제 활성

Antioxidant Activity and Inhibitory Effect on Oxidative DNA Damage of Ethyl Acetate Fractions Extracted from Cone of Red Pine (Pinus densiflora)

본 연구에서는 솔방울을 새로운 천연 식물자원으로서의 이용 가치를 평가하기 위해 솔방울 ethyl acetate 분획물의 항산화 활성과 DNA 손상억제 효과를 조사하였다. 솔방울 ethyl acetate 분획물의 DPPH, Fe²⁺+ 킬레이팅 및 환원력 활성은 대조군인 L-ascorbic acid와 유사한 활성을 나타내었다. 특히 ABTS 소거 활성의 IC50값은 L-ascorbic acid (10.49 ㎍/㎖)에 비해 솔방울 ethyl acetate 분획물(7.80 ㎍/㎖)이 더욱 높은 활성을 보였다. 또한 총페놀성 화합물과 비타민 C 함량은 각각 27.3 ㎎/g, 1.8 ㎎/g로 나타났다. Hydroxyl radical과 fenton reaction에 의한 산화적 DNA 손상억제 효과는 모든 농도에서 50% 이상의 높은 손상 억제 효과를 나타냈다. 따라서 본 연구결과를 통한 솔방울 ethyl acetate 분획물의 항산화 활성과 산화적 DNA 손상 억제 효과 입증은 다소 부가가치가 낮게 평가되어온 솔방울은 식품이나 천연 의약품 분야에 새로운 천연소재로써의 활용가치가 높을 것으로 판단된다.

Antioxidant activity and inhibitory effect on oxidative DNA damage of ethyl acetate fractions extracted from Cone of Red Pine (Pinus densiflora) were investigated to find utilization of Cone, by-product of Red Pine, thrown out after berry shatter, as a new natural plant resource. Cone from P. densiflora was extracted with methanol (MeOH) and separated to petroleum ether, ethyl acetate and water fraction. Among them, ethyl acetate fraction was used. The antioxidant activity was conducted by the 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical, 2, 2 -Azino-bis (3-ethylbenzothiazoline-6 sulfonic acid) diammonium salt (ABTS) radical scavenging assay, Fe²⁺+ chelating assay and reducing power assay. The inhibitory effect on oxidative DNA damage was determined by DNA cleavage assay using φX-174 RF I plasmid. The results of DPPH and ABTS radical scavenging activity at 200 ㎍/㎖ of extracts were 86.50% and 95.80% respectively, which were similar figures compared with L-ascorbic acid as control. Fe²⁺+ chelating activity was 77.96% and reducing power was 0.77 at 200 ㎍/㎖. Total phenolic component was 27.29±0.3 ㎎/g and Vitamin C content was 1.84±0.1 ㎎/g. Also ethyl acetate fraction from Cone has inhibitory effect, using φX-174 RF I plasmid on DNA cleavage assay. In conclusion, Cone, by-product of P. densiflora, showed high antioxidant activity and inhibitory effect on oxidative DNA damage. Therefore this study suggests Cone, useless by-product, can be developed as a new natural plant resource with lots of utilization such as an effective antioxidant, natural medicine, food, cosmetics and so on.