단양쑥부쟁이에 함유된 Caffeoylquinic Acid의 HPLC 분석과Peroxynitrite 소거효과

HPLC Analysis on the Cafffeoylquinic Acids of Aster altaicus var. uchiyamae Kitamura and Peroxynitrite-scavenging Effect

단양쑥부쟁이의 caffeoylquinic acid 성분을 HPLC로 분석하였다. 그 결과 3,4-DQ, 3,5-DQ, 4,5-DQ, 5-CQ, 3-CQ 5종 화합물이 검출되었다. 이 식물의 caffeoylquinic acid 총 함량은 26.73 ± 0.26 ㎎/g으로 건물중의 2.673 ± 0.026%, MeOH 추출물 중 함량은 25.22 ± 0.25%에 달하였다. 그리고 peroxynitrite 를 소거하는 IC50 값은 5.16 ± 0.15 ㎍/㎖로 나타났고, 양성대조군으로 사용된 L-penicillamine의 값은 0.89 ± 0.22 ㎍/㎖였다. 그러므로, 단양쑥부쟁이는 caffeoylquinic acid 함량에 따르는 peroxynitrite 소거효과에 기인하여 비만, 당뇨, 심혈관질환, 동맥경화 등에 유효할 것으로 예상된다.

Five caffeoylquinic acids of Aster altaicus var. uchiyamae Kitamura (Compositae) leaves were identified using standard compounds by HPLC and determined as follows: 3,4-di-O-caffeoylquinic acid (4.92 ± 0.06 ㎎/g dried weight), 3,5-di-O-caffeoylquinic acid (3.95 ± 0.13 ㎎/g), 4,5-di-O-caffeoylquinic acid (1.39 ± 0.10 ㎎/g), 5-O-caffeoylquinic acid (chlorogenic acid, 8.05 ± 0.21 ㎎/g), 3-O-caffeoylquinic acid (4.97 ± 0.18 ㎎/g). The total content of five caffeoylquinic acids were calculated as 26.73 ± 0.26 ㎎/g dried weight while the percentage of the five compounds in the MeOH extract was calculated as 25.22 ± 0.25%. The IC50 value of the MeOH extract scavenging peroxynitrite (ONOO - ) was shown as 5.16 ± 0.15 ㎍/㎖.