LED광원에 따른 감초 지상부의 생리활성 및 성분 평가

A Comparative Study of Physiological Activity and Ingredient Analysis of Glycyrrhiza uralensis Fischer Stems and Leaves Cultivated with Different Wavelength of LED Lights

LED 광원을 달리하여 재배한 감초지상부의 항산화, 항돌연변이원성을 평가하였다. BL-0에서 DPPH 라디칼 소거활성에서 EC50이 3.02 ± 0.13 ㎍/㎖와 ABTS 라디칼 소거활성은 EC50이 2.18 ± 0.18 ㎍/㎖와 환원력이 가장 높게 나타났다. 폴리페놀함량은 2.93 ± 0.11 g/100 g으로 가장 높게 나타났으며, RL-0에서는 항산화 활성이 가장 낮게 나타났다. 항돌연변이원성 평가에서 직접변이원인 1-NP에 대해 모든 실험군에서 돌연변이억제효과를 나타냈으며, 간접변이원인인 Trp-P-1, Trp-P-2,AFB1에 대해서는 BL-0에서 가장 높은 돌연변이 억제효과를 나타냈고, 2-AA에서는 RL-1에서 돌연변이 억제가 높게 나타났다. 청색광 LED (650 ㎚)와 적색광 LED (450 ㎚)를 이용하여 재배한 감초지상부의 성분변화를 관찰하기 위해 GC/MS를 이용해 분석한 결과 25분에서 RL-1에서만 cytidine이 검출되었고,37분에서 BL-0에서만 2-bromotrimethylene glycol이 특정적으로 검출되었다. 이는 LED광원 조건에 따라 감초 경엽의 성분이 차이가 있음을 보여준다. 이 성분들이 생리활성에 어떤 영향을 주는지는 더 검토해야할 부분이다.

Glycyrrhiza uralensis Fischer is one of the most commonly used herbs. Recently, the stem and leave of the planthave been interested in physiological activities because the aerial parts have been thrown away. Finding out cultivationmethod of Glycyrrhiza uralensis Fischer to improve chemical ingredients and biological activities has been tried these days. In this study, different wavelengths of light emitting diode (LED) were used for a cultivation of Glycyrrhiza uralensisFischer. Antioxidant activities and inhibitory effect on mutagenecity of samples were evaluated. The stem and leave cultivatedunder blue light (BL-0) showed the strongest antioxidant activities of 3.02 ± 0.13 ㎍/㎖ (EC50) and 2.18 ± 0.18 ㎍/㎖ (EC50)in DPPH and ABTS radical scavenging test, respectively. Total phenolic content of BL-0 was 2.93 ± 0.11 g/100 g, thehighest value between cultivation conditions. However, antioxidant activities of the stem and leave cultivated under redlight were the weakest between samples. All of the stem and leave used in this study showed inhibitory effect onmutagenecity of 1-nitropyrene. BL-0 showed stronger inhibitory effects on mutagenicity of Trp-P-1, Trp-P-2, and AFB1than samples cultivated under other conditions. Only on mutagenecity of 2-aminoanthracene, the stem and leave cultivatedat 1 m apart from red light (RL-1) showed the strongest inhibitory effect. These results indicate that blue LED might be themost effective condition for improvement of physiological activities for the aerial parts of Glycyrrhiza uralensis Fischer incultivation. The components were identified with GC/MS. Cytidine was detected only in RL-1 at 25 min of retention timeand 2-bromotrimethylene glycol was detected only in BL-0 at 37 min.