ICR웅성마우스에 5% DSS로 궤양성 대장염을 유발시킨 후, 정상대조군, 궤양유발군, 양성대조군 및 식방풍 잎 추출물 투여군의 궤양성 대장염에 미치는 효과를 요약한 결과는 다음과 같다. 1. 식방풍 잎 추출물 투여군은 양성대조군에 비해 체중감소 완화 효과를 나타냈지만, 농도에 따른 유의적인 차이는 나타나지 않았다. 2. 식방풍 잎 추출물 투여군의 대장조직 및 비장변화는 양성대조군과 비슷한 개선효과를 나타냈다. 3. 식방풍 잎 추출물 투여군은 혈중내 염증성 사이토카인을 농도 의존적으로 감소시켰으며, 대장조직내 염증성 사이토카인 mRNA 발현을 억제하는 효과를 나타냈다.
In general, Reucedani Radix (Peucedanum japonicum Thunbergis: PJ) is the Korean traditional herbal medicine used to remove dampness, to relieve pain, and spasm. So, PJ folium is believed to have the same effects. The aim of this study is to investigate the alleviation of dextran sulfate sodium (DSS) induced ulcerative colitis in mice by PJ folium. 25 mice were divided into 5 groups: normal, DSS, DSS + 100 ㎎/㎏ PJ folium (PJ100), DSS + 500 ㎎/㎏ PJ folium (PJ500), and DSS + 150 ㎎/㎏ 5-amino salicylic acid (5-ASA) groups. Body weights, colon lengths, histological changes in colon tissue, and spleen weights were observed. Inflammatory cytokines such as IL-1β, IL-6, and TNF-α level in plasma were measured by ELISA. IL-1β, IL-6, and TNF-a mRNA expression in colon tissue were detected by RT-PCR. In the results, body weight lose was inhibited in PJ100, PJ500, and 5-ASA groups, but it was not different compared with DSS group, significantly. PJ500 group showed the preventive effects of colon length shorten and histological changes in colon tissues as good as 5-ASA group. The weight of spleen was increased in DSS group but it reduced in PJ100, PJ500, and 5-ASA groups. Moreover, IL-1β and TNF-α cytokine levels in plasma were reduced in PJ500 and 5-ASA groups. IL-1β, IL-6, and TNF-a mRNA expression in colon tissue were inhibited in PJ100, PJ500, and 5-ASA groups and it was significantly different compared with DSS group. In conclusion, PJ folium showed the alleviative effect on DSS induced ulcerative colitis in mice.
재료 및 방법