물레나물 약배양에 의한 부정 신초 및 식물체 재분화

Adventitious Shoot and Plant Regeneration from AntherCulture of Hypericumascyron L.

In order to investigate the effects of low temperature pretreatment of floral bud and plant growth regulators on anther-derived callus and shoot differentiation, anthers were cultured on 1/2 MS medium supplemented with 2,4-D, NAA, BA and TDZ. This plant depends on the plant growth regulators, for these anthers couldn’t respond on 1/2 MS medium without plant growth regulators. 2,4-D was a prerequisite substance in this experiment, especially 52.6% of callus formation on MS medium with 2.0 mg/L 2,4-D alone. However, the optimum medium was on 1/2 MS medium with 0.1 mg/L 2,4-D and 1.0 mg/L BA for continuous growth and shoot differentiation from the anther. Calli derived from on MS medium with 2.0 mg/L 2,4-D transferred to the 1/2MS medium with TDZ and BA. TDZ were less superior to BA, only one anther could produce shoot onMS media with 1.0 mg/L TDZ. On the other hand, when the calli transferred to the medium with 3.0 mg/L BA, adventitious shoots were proliferated, subsequently, regenerated shoots elongated from the embryogenic calli. After floral buds of one week before anthesis were incubated at 5℃refrigerator for eight or fifteen days, anthers seperated from floral buds were cultured on 1/2 MS medium supplemented with 0.1 mg/L 2,4-D and 1.0 mg/L BA. Callusing and shoot differentiation on anthers from treated at 5℃for eight days were more effective than those of fifteen days or control.