새우난초(Calanthe discolor Lindl.)와 금새우난초[C. discolor for. sieboldii (Decne.) Ohwi]의 종간교배에 의한 1대잡종 종자의 기내무균 발아

Aseptic Germination of F1 Hybrid Seed by Inter-species Pollination of Calanthe discolor Lindl. and C. discolor for. Sieboldii (Decne.) Ohwi

Two orchid species of Calanthe discolor Lindl. and C. discolor for. sieboldii (Decne.) Ohwi, which have different form flower color and size. They were crossed in mid April by artificial pollination, and the F1 hybrid seeds were collected mid October. Germination of seeds was investigated on pre-treatment of seeds and under the various environmental conditions. Germination was promoted by moisture absorption and ultrasonic treatment of seeds. Dark culture of F1 hybrid seeds enhanced germination and protocorm formation, and development into seedlings compared with light culture. Although, plant growth regulators such as NAA and BA had a slightly promotive effect on seed germination and protocorm growth, regenerated seeding were showed abnormal growth patterns. Regenerated F1 hybrid plantlets were successfully transferred to pot.