Two-year-old seedlings of Ilex rotunda were grown under control (full sunlight) and three different shading condition. Those conditions were full sunlight (PPFD 1600μmol m-2 s-1), 30% (PPFD 400μmol m-2 s-1), 50% (PPFD 250μ mol m-2 s-1) and 70% treatment (PPFD 100μmol m-2 s-1). Total chlorophyll contents were inverse proportion to light intensity. Seedlings under full sunlight showed the highest photosynthetic activity such as photosynthetic rate, intercellular CO2 concentration and water use efficiency. Photosynthetic activity trend was increased at the higher light intensity than the lower treatment over PPFD 500μmol m-2 s-1. Especially, seedlings under 70% treatment showed the worst photosynthetic activity at high light intensity. That result was regular for adapted plant in low intensity environment. Leaf area was also inverse proportion to light intensity, while dry weight per leaf area was shown the opposite trend.
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