고추 유전자변형체 후대 생육특성 검정

Characteristic of Progeny in Pepper Transformants

For the resistance test for Phytophthora blight of T1 and T2 transformants in pepper, Phytophthora blight fungus was inoculated to seedlings of the T1 and T2 transformants by concentration (density: zoospore 103/ml). Occurrence rate of blight at 5days after inoculation was 4.0 % in T1-1 line and 10.0% in T1-2 line, and its rate for 12 days after inoculation was 52.0% in T1-1 line, 64.0% in T1-2 line, respectively. Therefore, the lower occurrence rate to blight was enable to select resistant transformants in the some inoculation density (zoospore 103/ml), meanwhile Kumtap and Subicho were 100% in highest occurrence rate to blight. For field test, in which blight was commonly occurred, of the Youngyang Pepper Experiment Station, the acquired transformant resisting to blight was similar to characteristics of domestic varieties, Subicho for fruit shape, but there are some differences in growth, days to flowering, fruit characteristics. Occurrence of blight in T2-1-6, and T2-4-9 lines was smaller approxmately 30% than commercial varieties, Kumtap , although occurrence of blight in field was showed higher difference among tested lines. In this study, we concluded that the transformants showing blight resistance selected from habitual field could be fixed at every generation, and the developed transformation system was also considered to develop transformants in pepper.