단마와 장마 영여자의 항산화능 및 항돌연변이 활성 검정

Evaluation of Antioxidant Capacity and Antimutagen Activity of Bulbil Extracts of the Dioscorea japonica Decaisne and Dioscorea batatas Decaisne

단마와 장마 영여자에 대한 항산화 및 항돌연변이원성을비교･평가하였다. 단마와 장마 영여자 80% 메탄올의 폴리페놀함량은 2.2 와 3.9 mg/g로 장마에서 높았다. 또한 분획물에 있어서는 장마 영여자 에틸아세테이트 분획물에서가장 높은 함량을 나타냈다. 그리고 DPPH과 ABTS 라디칼소거능 및 지질과산화 억제효과에 있어서는 총폴리페놀이높은 장마와 에틸아세테이트 분획물에서 가장 높은 활성을나타냈다. 한편 단마와 장마 영여자 80%메탄올 추출물 1mg/plate의 1-NP, AFB1, Trp-P-1에 대한 항돌연변이원성은 S. typhimurium TA98(hisD3052)에서 37.5, 24.5,37.9% 돌연변이 억제효과를 나타내었으며, 장마 영여자는31.7, 53.9, 56.85%의 돌연변이 억제효과를 나타냈다. 또한 분획물 중에는 단마와 장마 영여자 클로로포름과 에틸아세테이트 분획물에서만 돌연변이 억제효과를 나타내었으며, 농도 의존적으로 증가하였다.

The bulbil of the Dioscorea species is produced, the amount of which is 2,000 tons annually, but it has been discarded without specific use. In this study the antioxidant and antimutagenicity of bulbil of the Dioscorea, which compared to bulbil of Danma(Dioscorea japonica Decaisne) and Jangma(Dioscorea batatas Decaisne), a major domestic cultivation species. The study was done by extracting bulbil of the Dioscorea methanol and the methanol extracts was re-extracted with chloroform, ethyl acetate, n-butanol and water. In methanol extract of Danma and Jangma, polyphenolic compounds contained 2.2 and 3.9 mg/g extract, respectively. The ethyl acetate fraction of Danma and Jangma had higher polyphenolic contents of 33.9 and 39.1 mg/g, whereas water fractions were much lower at 2.4 and 5.8 mg/g. Determination of antioxidant activity showed that the ethylacetate fraction strong DPPH radical scavenging activity and ABTS radical scavenging activity. The inhibitory effects of methanol extracts and chloroform, ethylacetate, n-butanol, water fraction from bulbil of Danma and Jangma on the mutagenicity in 1-NP, AFB1, Trp-P-1 were investigated using S. typhimurium TA98. Danma and Jangma cultivars decreased the reverse mutation induced by 1-NP, AFB1, Trp-P-1 in S. typhimurium TA98. The fraction of chloroform and ethylacetate showed strong inhibitory effects, in a dose dependant manner against the mutagenicities induced by 1-NP, AFB1, Trp-P-1 in S. typhimurium TA98.