Chlorogenic acid, formed of an ester of caffeic acid and quinic acid, which is naturally abundant in many plant species, was used as a model O-dihydoxy phenolic compound. In the previous study, we have reported that the isolated constituent from Apocynum venetum leaves has an inhibitory effect on Cu2+ - induced oxidative modification of low-density lipoprotein(LDL). Among them, chlorogenic acid showed the most potent anti-LDL oxidative activity than other compounds. For the reasons, we are investigated the inhibitory effect of the chlorogenic acid on Cu2+ - induced oxidative modification of LDL, monitored a lag time in the conjugated-diene formation and TBARS formation, and measured TNBS free amino acid group, and form cell formation in vitro system. The TBARS- and diene- formation were strongly inhibited by chlorogenic acid (0 – 100 ㎍ / ml) with dose dependent manner. On the other hand, TNBS reactive lysine amino groups on LDL oxidation were protected by chlorogenic acid- treated cell group. Therinforth, chlorogenic acid was inhibited to cholesterol accumulation in the isolated peritoneal macrophage.
Materials and Methods