개머루덩굴 추출물의 항산화 효과

The antioxidative effects of Ampelopsis brevipedunculata extracts

본 연구에서는 개머루덩굴 95% 에탄올추출물의 항산화 효과를 조사하였다. Pyrogallol의 억제율을 100%로 기준하였을 때, DPPH 라디칼을 50% 억제시키는데 필요한 개머루덩굴 추출물의 농도는 0.42 mg/mL이었다. 총항산화 활성은 ABTS 라디칼에 대한 항산화능으로 측정하였다. 개머루덩굴 추출물 0.1 및 1 mg/mL의 총항산화능은 각각 0.65 및 3.71 mM Trolox와 동등한 수준이었다. 개머루덩굴 추출물 5 및 100 μg/mL의 peroxyl radical 소거능은 각각 22.75 및 131.25 μM gallic acid와 동등한 수준이었다. 개머루덩굴 추출물 0.1 및 1 mg/mL의 superoxide 소거능은 각각 27.7 및 56.0%이었다. 개머루덩굴 추출물 0.5 및 2 mg/mL의 총페놀 함량은 각각 0.55 및 2.06 mM gallic acid와 동등한 수준이었다. 또한, HepG2 세포주를 이용한 세포배양에서 개머루덩굴 추출물 0.1 mg/mL 농도의 첨가는 0.2 및 0.5 mM tert-butyl hydroperoxide로 유도된 세포독성을 각각 36.2 및 23.3% 감소시켰다. 따라서, 본 연구 결과들은 개머루덩굴 추출물의 강력한 항산화 효과와 세포독성 억제 효과를 나타내며, 이러한 효능은 적어도 자유라디칼의 산화 억제와 높은 총페놀 함량에 기인하는 것으로 사료된다.

This study was carried out to investigate the antioxidative capacity of Ampelopsis brevipedunculata 95% ethanol extracts. The concentration of A. brevipedunculata extract at which DPPH radical scavenging activity was inhibited by 50% was 0.42 mg/mL as compared to 100% by pyrogallol as a reference. Total antioxidant status was examined by total antioxidant capacity against ABTS radical reactions. Total antioxidant capacities of A. brevipedunculata extract at the concentrations of 0.1 and 1 mg/mL were 0.65 and 3.71 mM Trolox equivalents, respectively. Oxygen radical absorbance capacities of A. brevipedunculata extract at the concentrations of 5 and 100 μg/mL were 22.75 and 131.25 μM μM gallic acid equivalents, respectively. Superoxide scavenging activities of A. brevipedunculata extract at the concentrations of 0.1 and 1 mg/mL were 27.7 and 56.0%, respectively. Total phenolic contents of A. brevipedunculata extract at the concentrations of 0.5 and 2.0 mg/mL were 0.55 and 2.06 mM gallic acid equivalents, respectively. A. brevipedunculata extract at the concentration of 0.1 mg/mL inhibited 0.2 mM and 0.5 mM tert-butyl hydroperoxide induced cyototoxicity by 36.2 and 23.3%, respectively, in HepG2 cell culture system. Thus strong antioxidant and cytotoxicity-inhibiting effects of A. brevipedunculata extract seem to be due to, at least in part, the prevention from free radicals-induced oxidation as well as high levels in total phenolic contents.