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KCI등재 학술저널

Cryopreservation of Somatic Embryos of Soapbeny (Sapindus mukorossi Gaertn.) by Vitrification

Cryopreservation of Somatic Embryos of Soapbeny (Sapindus mukorossi Gaertn.) by Vitrification

Somatic embryos do not survive at exposure to liquid nitrogen temperatures without cryoprotective treatments. A simplified technique which simultaneously induces and cryoprotects embryogenic calli using plant vitrification solution 2 (PVS2) followed by dehydration was developed for the cryopreservation of Soap berry genetic resources. Vitrification is a way of removing the moisture in vegetation through PVS2. The PVS2 vitrification solution consisted of 30% glycerol (w/v), 15% ethylene glycol (w/v), 15% Dimethylsulfoxide (w/v) in B5 medium containing 0.4M sucrose. Two tests were done. The one was to eliminate moisture at 0∘C and the other at 25∘C. In both cases the best results came out at a vitrification time of 10∼20 minutes. It was also found that the survival rate was higher at 0∘C than at 25∘C. In particular, the survival rate reached more than 80%. Water-damaged embryos turned brown and stoped growth, but energetic embryos took on a milky hue and show a very vigorous growth rate. Successful cryopreservation of somatic embryos of soapberry can be used to establish in vitro genebanks for long-term conservation of Soapberry genetic resources to complement field genebanks and other in vitro methods already being used.

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