Production of Transgenic Petunia hybrida cv. Rosanpion Using Agrobacterium-mediated Transformation

Transgenic Petunia hybrida cv. Rosanpion was produced by Agrobacterium tumefaciens LBA4404 harboring a binary vector PBI 121 containing B-glucuronidase (gus) and neomycin phosphotransferase (nptII). For genetic transformation, leaf discs were precultured on MS medium supplemented with 0.5 mg/L NAA and 1.0 mg/L BA (MNB) for 2 days and cocultured for 15 mins with A. tumefaciens. For selection of transformant, leaf discs were transferred to fresh MNB containing 50 mg/L kanamycin and 500 mg/L cefotaxime. Eighteen plants were regenerated and four were confirmed by PCR for detection of gus and nptII gene integrated into the nuclear genome of petunia Rosanpion . Using this transformation system, we expect that transgenic petunia Rosanpion incorporating a useful gene can be produced.