In Vitro Morphogenesis through Leaf Explants of Gypsophila paniculata L.

Callus cultures from leaf explants of Gypsophila paniculata L. cv. Bristol Fairy have been tested their growth and morphogenic capacity on Murashige and Skoog medium supplemented with 0.1, 0.5, 1 and 3 mg/L 2,4-D. The frequency of callus formation ranged from 43.3% to 100%. The optimal 2,4-D concentration for promoting callus formation and growth was 0.5 to 3 mg/L. 4.2~5.6% of adventitious roots were obtained with the use of 0.1 and 0.5 mg/L 2,4-D. Calli grown well on 1.0 mg/L 2,4-D was the heaviest among the calli grown in various concentrations.