AFLP marker를 이용한 콩의 유전적 다양성과 유전분리 분석

Diversity and Inheritance of AFLP Markers in Wild and Cultivated Soybeans

AFLP marker의 유용성 을 알아보고자 재배콩과 야생콩을 대상으로 유전적 다양성과 유전분리 현상을 분석하였다. 공시 재료들의 polymorphism은 재배 콩과 야생 콩에서 각각 평 균 2 9%와 12.2%의 polymorphism을 보였으며, 재배 콩과 야생 콩에서 공히 유전적 다양성을 보인 DNA단편은 11개 primer 평균 24개를 나타내었다. Primer 조합별로도 polymorphism에 다양한 차이가 있었는데 평균 22.9%로 13.0-38.5%의 변이를 나타내었다. 재배 콩 간의 교잡후대(화엄풋콩 × PI417479) F₂집단에서 AFLP marker의 유전분리 양상을 분석한 결과 3 : 1의 분리 비를 따르는 것으로 판단되었다.

Genetic variation is the basis of crop improvement. Limited genetic diversity in a crop species may restrict the amount of genetic improvement that can be achieved through plant breeding. Soybean is one of the world s most important crops. A potential source of genetic variability for the cultivated soybean is the wild species G. soja Sieb. ＆amp; Zucc. Amplified fragment length polymorphism (AFLP) analysis is a PCR-based technique, which can detect a 10-fold greater nubmer of loci than other DNA marker analysis. Twenty cultivated soybeans and two-hundred wild soybeans were used to determine genetic vatiations by AFLPs and evaluate the usefulness of AFLPs as DNA markers. Six-hundred and ten fragments were detected with an average of 56 AFLP fragments produced per primer in a total of 11 AFLP primer pairs. The number of polymorphic loci detected per primer ranged from 7 to 20 and the polymorphism was greater in wild than in cultivated soybean. F₂ segregation analysis of four AFLP fragments in combination of Hwaeomputkong × PI 417479 indicated that they segregate as stable Mendelian loci with 3 : 1. This results strongly suggest that the AFLP analysis is a good technique for the detection of genetic polymorphism in a wide plant species.