Variation of Iron Content and Ferritin Distribution during Development Stage under Conditions of Iron Nutritional Status from Hydroponic Culture in Red Pepper(Capsicum annuum L.)
Total iron content and ferritin distribution have been determined in red pepperi Cupsicum annum L.) during development stage under conditions of iron nutritional status from hydroponic culture. Color of the leaves become chlorotic on iron deficient and high concentration. The plant height on each iron concentration had retarding effect at concentration lower than 254M and greater than 1254M. In normal green leaves, total iron content was almost constant with a mean value of 2.5pmole of iron/mg of dry matter, except at 63day, for which it increases slightly to tumole. However, iron content of chlorotic plants grew on iron free medium was not almost detectable. Also, In post chlorotic leaves(++Fe), iron content was evidently increased until 7days after transfer on liquid medium, but decreased from after 14days. Also, ferritin protein analysed total protein extracts prepared from leaves of different ages using antibodies raised against ferritin protein. Ferritin protein decreased progressively during the first week of germination and was not detectable in vegetative tissues. Ferritin protein in post chlorotic leaves was evidently strongly enhanced until lidays afier transfer on liquid medium but decreased until the leves hecame chlorotic.
MATERIALS AND METHODS