RGP렌즈와 역기하렌즈가 가토각막의 상피세포증식률에 미치는 영향

The Effect of RGP Lens and Reverse Geometry Lens on Corneal Epithelial Proliferation Rate in Rabbit

목적: RGP와 역기하렌즈착욕이 가토 각막상피세포증식률에 미치는 영향을 알고자 하였다. 대상과 방법: 기토 24마리에 우안은 RGP나 역기하렌즈를 착용시키고 좌안은 대조안으로 사용하여 렌즈착용 후 1, 3, 7, 14일에 각각 3마리씩 희생하였다. 5-bromo-2-deoxyuridine를 이용하여 면역형광염색 후 상부각막윤부에서 중심부까지 1.0 mm간격으로 나누어 광학현미경상 200배 배율에서 각막상피에 염색된 세포를 관찰하였다. 결과: 정상가토의 각막은 윤부에서 염색된 세포가 가장 적었고, 주변부각막에서 가장 높게 염색되었다(p<0.05). RGP렌즈 착용 1일 후 염색률이 각막윤부에서 127%증가하였고, 중심부는 24%감소하였다. 역기하렌즈 착용 1일 후 주변부와 중심부는 12%, 121%증가하였으나 윤부는 16%감소하였고, 7일 후는 윤부와 중심부 모두 감소하였다. 착용 14일 후 RGP와 역기하군 모두 윤부에서 8%로, 중심부는 32%, 63%로 감소하였다. 결론: 정상가토각막에서 보인 위치에 따른 다양한 증식률의 분포는 RGP와 역기하렌즈를 착용 후에 변화하였고, 역기하렌즈군에서 변화가 더 심하였다. 이는 역기하렌즈가 RGP렌즈보다 덜 생리적임을 시사한다.

Purpose: To investigate proliferation rate of the corneal epithelium with rigid gas permeable lens(RGP) or reverse geometry lens(RGL) wearing. Methods: Twenty-four rabbits were fitted with either a RGP or RGL on right eye, with left eye serving as a control. They were sacrificed at 1, 3, 7, and 14 days after lens fitting. after immunohistochemistry using 5-bromo-2-deotyuridine, BrdU-labeled cells were counted in 10-medium power fields (×200) in each sample using light microscope by 1.0 mm intervals between superior limbus and the center. Results: The number of BrdU-labeled cells was highest in peripheral cornea, and lowest in limbus(p<0.05). The BrdU-labeled cells of limbus increased by 127% but labeled-cells of the center decreased by 24% in RGP group after 1-day fitting. Increase of the labeled cells was observed in the mid-peripheral and central cornea by 12% and 121% each but the decrease of labelled cells was found at the limbus by 16% in RGL fitting group at 1 day. After 14days, Both RGP and RGL group showed decrease of BrdU-labeled cells in limbus by 8% each, and in the center by 32% and 63%, respectively. Conclusions: Normal rabbit cornea was characterized by different epithelial proliferation rates according to location. However these pattern was significantly altered with both RGL and RGP fitting group. The change was greater in RGL group than in RGP group. This suggested wearing the RGL might be less physiologic than RGP fitting.