전라남도산림자원연구소 추출물은행 데이터 구축을 위한 난대수종의 추출물 분석 연구

Warm Temperate species extract Analysis for Jeollanamdo Forest Resources Institute extracts Bank Data building

This study developed a HPLC analysis method for the determination of avicularin (AL) and quercitrin (QT) in Astilbe chinensis by HPLC. HPLC was performed on a Capcell Pak C18 MGII column (<TEX>$4.6{\times}250mm$</TEX>, <TEX>$5{\mu}m$</TEX>) with a gradient elution of 0.05% (v/v) trifluoroacetic acid (TFA) and acetonitrile at a flow rate of 1.0 mL/min at <TEX>$30^{\circ}C$</TEX> and a UV detection wavelength of 254 nm. The analytical method was validated for its specificity, precision, accuracy, and linearity. The limits of detection and quantitation were AL 0.09 and 0.28 mg/mL, QT 0.03 and 0.09 mg/mL respectively. Calibration curves showed good linearity (AL r2 > 0.99990 and QT r2 > 0.99994), and the precision of analysis was satisfied (less than AL 0.59%, QT 0.63%). Recoveries of quantified compounds ranged from AL 100.57 to 102.08%, QT 99.94 to 100.64%. These results indicate that the developed HPLC method was applied successfully to the detection of AL and QT in A. chinensis.