Studies on the Appropriate Light Environment and Concentration of Growth Regulators for the Culture of Flower Pot Plants of a Jeju Native Plant, Polygonatum odoratum D.

Studies on the Appropriate Light Environment and Concentration of Growth Regulators for the Culture of Flower Pot Plants of a Jeju Native Plant, Polygonatum odoratum D.

The goal of this research was to develop the effectiveness of in vitro culture method for A. formosanus and study the environment in vitro conditions affecting on growth. The first series of experiments were examined to investigate the response of three different basal media, MS (Murashige and Skoog, 1962), Knudson (KC; Knudson, 1946) and modified hyponex on growth and multiplication during in vitro culture. Multiple shoot proliferation was induced in shoot tip explants on Hyponex (H3) media supplemented with BA (1 mg1<TEX>$\^$</TEX>-1/) or TDZ (1-2 mg1<TEX>$\^$</TEX>-1/). Addition of activated charcoal (1%) to the TDZ containing medium promoted rapid shoot tip proliferation (11.1 shoots per explant) but the same medium had an opposite effect resulting in poor proliferation in the nodal explants. However, the regenerated shoots had slow growth rate and failed to elongate. This problem was overcome by transferring the shoot clumps to a hormone free H3 media supplemented with 2% sucrose and 0.5% activated charcoal. Using bioreactor culture for scaling up was also shown the best way for multiple shoot induction and growth of this plant. The second series of experiments was studied to investigate the effect of physical environment factors on growth of in vitro plantlets. The Anoectochilus formosanus plantlets were cultured under different air exchange rate (0.1, 0.9, 1.2h<TEX>$\^$</TEX>-1/), without sucrose or supplement 20g.1<TEX>$\^$</TEX>-1/ (photoautotrophic or photomixotrophic, respectively), and different photosynthesis photon flux (40, 80, 120 ,<TEX>${\mu}$</TEX>mol.m<TEX>$^2$</TEX>.s<TEX>$\^$</TEX>-1/- PPF). Under non-enrichment CO<TEX>$_2$</TEX> treatment, slow growth was observed in photoautotrophical condition as compared with photomixotrophical condition on shoot height, fresh weigh and dry weight parameters; High air exchange (1.2.h-l) was found to be inadequate for plant growth in photomixotrophical condition. On the contrary, under CO<TEX>$_2$</TEX>, enri