섬쑥부쟁이로부터 건강기능식품 제조를 위한 추출법 개발

Development of the Extraction Method for a Functional Food from Aster glehni

In this study, we analyzed 80%MeOH extract of fruits of sorbaria sorbifolia var. stellipila MAX. to measure the total antioxidant capacity by oxygen radical absorbance capacity (ORAC) assay, individual flavonoid content by high-performance liquid chromatography (HPLC). n-Hexane (<TEX>$1.02{\pm}0.036$</TEX>), <TEX>$CH_2Cl_2$</TEX> (<TEX>$0.95{\pm}0.025$</TEX>), EtOAc (<TEX>$1.94{\pm}0.065$</TEX>), n-BuOH (<TEX>$1.98{\pm}0.054$</TEX>), D.W. (<TEX>$1.2{\pm}0.032$</TEX>) fractions were examined antioxidative activity by ORAC assay. It was revealed that EtOAc(<TEX>$1.94{\pm}0.065$</TEX>), n-BuOH(<TEX>$1.98{\pm}0.054$</TEX>) fractions had significant antioxidative activity. The isolation and separation were facilitated using open column chromatography, while separation, purification and identification were accomplished by using high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR).