Anti-inflammatory Metabolites of Agrimonia pilosa Ledeb. and Their Mechanism

Anti-inflammatory Metabolites of Agrimonia pilosa Ledeb. and Their Mechanism

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This Camptotheca acuminata Decne. (CA), belonging to Nyssaceae, is a deciduous tree. and has been used as Traditional Chinese medicine since ancient times. The CA produces camptothecin a natural indole alkaloid, and reported to have anti-cancer effects. But the studies on biological activities of CA leaves are insufficient. Therefore, this study confirmed various biological activities such as antioxidant, antidiabetic, anticancer, antiinflammatory and metabolism analysis by HPLC-MS/MS of CA leaves. The <TEX>$RC_{50}$</TEX> values of DPPH radical scavenging activity of ethyl acetate fraction, n-Butanol fraction, methanol extraction, water fraction and n-Hexane fraction were <TEX>$12.23{\pm}0.01$</TEX>, <TEX>$15.93{\pm}0.42$</TEX>, <TEX>$55.12{\pm}0.45$</TEX>, <TEX>$56.29{\pm}4.15$</TEX> and <TEX>$427.29{\pm}6.13ug/mL$</TEX>, respectively. The <TEX>$IC_{50}$</TEX> values of <TEX>${\alpha}$</TEX>-glucosidase inhibitory activity of ethyl acetate fraction, n-Butanol fraction, methanol extraction, n-Hexane fraction and water fraction were <TEX>$24.29{\pm}0.14$</TEX>, <TEX>$47.86{\pm}0.45$</TEX>, <TEX>$54.23{\pm}1.21$</TEX> <TEX>$466.76{\pm}2.21$</TEX> and <TEX>$623.91{\pm}9.67ug/mL$</TEX>, respectively. The nitric oxide release activity of n-Hexane fraction, methanol extraction, ethyl acetate fraction, water fraction and n-Butanol fraction were <TEX>$31.49{\pm}5.74$</TEX>, <TEX>$29.79{\pm}0.71$</TEX>, <TEX>$26.89{\pm}0.71$</TEX>, <TEX>$8.24{\pm}5.83$</TEX> and <TEX>$7.75{\pm}4.08%$</TEX> at 25 ug/mL, respectively. The anti-cancer activity of n-Hexane fraction, methanol extraction, ethyl acetate fraction, water fraction and n-Butanol fraction were <TEX>$31.49{\pm}5.74$</TEX>, <TEX>$29.79{\pm}0.71$</TEX>, <TEX>$26.89{\pm}0.71$</TEX>, <TEX>$8.24{\pm}5.83$</TEX> and <TEX>$7.75{\pm}4.08%$</TEX> at 25 ug/mL, respectively. The ethyl acetate fraction activities showed higher biological activities than other fractions. Thus, Additional studies were conducted using ethyl acetate fr