어성초, 금잔화 추출물의 모유두세포와 지방전구세포에 대한 세포독성 및 발모효과

Cell Viability and Hair Growth Effect of Ethanol Extract from Houttuynia cordata Whole Plant and Calendula arvensis Flower on HDFDPC and 3T3-L1 Cells

Chlorogenic acid is a phenolic compound found in Cudrania tricuspidata fruits. In the present study, the effect of chlorogenic acid on the inhibition of adipogenesis in 3T3-L1 adipocytes was investigated. Cells were stained with Oil red O reagent to detect lipid droplets in adipocytes. The 3T3-L1 cells were lysed and measured for intracellular triglyceride and adipokine by ELISA kit. The protein expression of adipogenesis-related gene was evaluated by Western blot analysis. Chlorogenic suppressed lipid droplet and intracellular triglyceride accumulation in a concentration manner and also decreased secretion of adipokines such as leptin and adiponectin, compared with fully differentiated adipocytes. Treatment of 3T3-L1 cells with chlorogenic acid reduced the protein levels of peroxisome proliferator-activated receptor gamma (<TEX>$PPAR{\gamma}$</TEX>) and, CCAAT/enhancer binding proteins alpha (<TEX>$C/EBP{\alpha}$</TEX>). This indicates that chlrogenic acid was effective as an anti-obesity agent by repressing the differentiation of 3T3-L1 into adipocytes and inhibiting triglyceridef formation in adipocyte and that it exerts its role mainly through the significant down-regulation of <TEX>$PPAR{\gamma}$</TEX> and <TEX>$C/EBP{\alpha}$</TEX>.