Fruit Mediated Synthesis of Gold and Silver Nanoparticles Using Lycium chinense and Their Antimicrobial Activity
Fruit Mediated Synthesis of Gold and Silver Nanoparticles Using Lycium chinense and Their Antimicrobial Activity
- 한국자원식물학회
- 한국자원식물학회 학술심포지엄
- 2018년도 춘계학술발표회
- 2018.04
- 94 - 94 (1 pages)
Uric acid is the end product of purine metabolism in human body, originating from hypoxanthine after enzyme catalysis by Xanthine oxidase (XOD). Hyperuricemia results as a result of either over-generation of uric acid or a reduction in its excretion. In silico modelling methods such as Absorption, Distribution, Metabolism, Excretion and Toxicity (ADMET) prediction, Autodock 4.2.6 program were used to study the potential inhibitory compounds of XOD. Also we investigated the inhibition of XOD activity by using the extracts of Dendropanax morbifera and Rubus coreanus Miq spectrophotometrically. According to ADMET data, several compounds from D. morbifera and R. coreanus plants, were found to be more potent in inhibiting the XOD activity than allopurinol. XOD inhibitory activity is evaluated by quantifying the formation of uric acid by measuring the absorbance at 290 m (<TEX>$A_{290}$</TEX>).D. morbifera extract inhibited XOD activity at <TEX>$250{\mu}g/ml$</TEX>, however the extracts from R. coreanus has inhibited XOD activity at <TEX>$25{\mu}g/ml$</TEX>. The major compound of R. coreanus, ellagic acid significantly increased the inhibition rate from <TEX>$9{\mu}g/ml$</TEX> and showed a 71% suppression rate at <TEX>$15{\mu}g/ml$</TEX>. Finally, these results suggested a potential inhibitory activities of the extracts from D. morbifera and R. coreanus Miq, but further research is needed to validate to ensure their safe usage as drug.