Effects of Osmotic and Non-Osmotic Priming Methods for Enhancing Germination of Pepper Seed

Effects of Osmotic and Non-Osmotic Priming Methods for Enhancing Germination of Pepper Seed

In order to increase the germination speed and uniformity of tomato seeds, sonication and modified drum priming treatments were investigated to produce high quality seeds for export. Sonication treatment was performed for 5, 10 and 20minutes at an intensity of 5.2, 10.4 and 15.7kHz in water at <TEX>$15^{\circ}C$</TEX>. After sonication treatment, seeds were primed with water or 100mM <TEX>$KNO_3$</TEX> for 4days. 40, 50 and 60% seed moisture content (SMC) of hydrated seeds were incubated for 60, 72 and 84h in a container with a relative humidity of 99% at 26rpm for a modified drum priming treatments. Germination speed were highly improved by sonication with osmotic priming. The seed treatment of osmotic priming or hydro priming after sonication or sonication without priming enhanced germination percentage (GP) on the <TEX>$2^{rd}$</TEX>day after sowing to 46%, 43% and 28%, respectively, while untreated seeds resulted in only 1% GP. These treatments also highly improved mean germination time (MGT) to 1.4, 1.8 and 2.6days, respectively, when compared to 3.5days MGT of untreated seeds. The modified drum priming treatment (72h incubation after 60% SMC hydration) significantly improved results of 74% GP(on the <TEX>$3^{rd}$</TEX>day after sowing), 2.6days MGT and <TEX>$39%{\cdot}day^{-1}$</TEX> germination rate (GR), however, untreated seeds showed 19% GP, 4.1 MGT, and <TEX>$25%{\cdot}day^{-1}$</TEX> GR. Although osmotic priming after sonication, hydro priming showed similar improved germination characteristics, however, modified drum priming is considered as an industrially promising treatment methods considering the shortening of the treatment period and environment-friendly aspects.