The Branch Extracts of Vaccinium oldhamii Stimulate Melanin Synthesis Through Activation of Tyrosinase Activity in B16F10 Melanoma Cells

The Branch Extracts of Vaccinium oldhamii Stimulate Melanin Synthesis Through Activation of Tyrosinase Activity in B16F10 Melanoma Cells

Although the roots of Heracleum moellendorffii (HM-R) have been long treated for inflammatory human diseases, scientific evidence for the anti-inflammatory activity of HM-R is not sufficient. In this study, we investigated anti-inflammatory activity and mechanism of action of HM-R in LPS-stimulated RAW264.7 cells. HM-R blocked LPS-induced NO and PGE2 production, but not HM-L. HM-R inhibited LPS-induced overexpression of iNOS, COX-2, <TEX>$IL-1{\beta}$</TEX> and IL-6 in RAW264.7 cells. HM-R inhibited LPS-induced <TEX>$NF-{\kappa}B$</TEX> signaling activation through blocking <TEX>$I{\kappa}B-{\alpha}$</TEX> degradation and p65 nuclear accumulation. In addition, HM-R inhibited MAPK signaling activation by attenuating the phosphorylation of ERK1/2, p38 and JNK. Furthermore, HM-R inhibited attenuated LPS-mediated overexpression of the osteoclast-specific factors such as NFATc1, cathepsin K, MCP-1 and TRAP. These results indicate that HM-R may exert anti-inflammatory activity by inhibiting <TEX>$NF-{\kappa}B$</TEX> and MAPK signaling activation. From these findings, HM-R has potential to be a candidate for the development of chemopreventive or therapeutic agents for the inflammation and inflammatory diseases.