깔라몬딘오렌지로부터 집속초음파추출을 이용한 기능성 물질 생산 공정 최적화

Process Development for Production of Functional material from Citrus Madurensis(Citrofortunella microcarpa) Using Innovative Extraction by Focused Ultrasound(INEFU).

In this study, we investigated the effect of branch extracts from Vaccinium oldhamii (VOB) on melanin synthesis in B16F10 cells. VOB promoted melanin production in absence or presence of <TEX>${\alpha}$</TEX>-melanocyte-stimulating hormone (<TEX>${\alpha}-MSH$</TEX>) in B16F10 cells. However, VOB did not affect the expression of tyrosinase and TRP-1 associated with melanin synthesis at the mRNA and protein level in B16F10. But, VOB decreased TRP-2 protein level and induced tyrosinase activation in B16F10 cells. Inhibition of tyrosinase activity and tyrosinase knockdown attenuated VOB-mediated melanin synthesis. In conclusion, it is thought that VOB may stimulate melanin synthesis through activating tyrosinase activity.