Anticancer Activity of Sageretia theezans in Human Colorectal Cancer Cells

Anticancer Activity of Sageretia theezans in Human Colorectal Cancer Cells

Bacillus subtilis B22, a chemotrophic and aerobic bacterial strain was isolated from homemade kimchi, identified by 16S rRNA gene sequencing. B22 was primarily screened by biochemical, carbon source utilization tests. B22 was used to produce pectinase and <TEX>${\beta}$</TEX>-glucosidase by submerged fermentation under different light sources. B22 was incubated in pectin media and basal media (pH 7.0) under blue, green, red and white light-emitting diodes (LEDs), fluorescent white light, and in darkness at <TEX>$37^{\circ}C$</TEX>, orbital shaker 150 rpm for 24 hours. Fermentation under blue LEDs maximized pectinase production (<TEX>$71.59{\pm}1.6U/mL$</TEX> at 24 h) and <TEX>${\beta}$</TEX>-glucosidase production (<TEX>$56.31{\pm}1.6U/mL$</TEX> at 24 h). Further, the production of enzyme increased to pectinase (<TEX>$156{\pm}1.28U/mL$</TEX>) and <TEX>${\beta}$</TEX>-glucosidase (<TEX>$172{\pm}1.28U/mL$</TEX>) with 3% glucose as a carbon source. Activity and stability of the partially purified enzymes were higher at pH 6.0 to 8.0 and <TEX>$25-55^{\circ}C$</TEX>. The effect on the metal ions <TEX>$Na^+$</TEX> and <TEX>$K^+$</TEX> and (moderateactivity) <TEX>$Mn^{2+}$</TEX> and <TEX>$Ni^{2+}$</TEX> increased activity, while <TEX>$Hg^{2+}$</TEX>, <TEX>$Cu^{2+}$</TEX>, <TEX>$Fe^{2+}$</TEX>, and <TEX>$Fe^{2+}$</TEX> inhibited activity. EDTA, phenylmethylsulfonyl fluoride and 5,5-dithiobis (2-nitrobenzoicacid) reduced activity, while tetrafluoroethylene and 1,10-phenanthroline inhibited activity. The amylase was highly tolerant of the surfactants TritonX-100, Tween-20, Tween-80 and compatible with organic solvents methanol, ethanol, isoamylalcohol, isopropanol, t-butylalcohol and the oxidizing agents hydrogen peroxide, sodium perborate and sodium hypochlorite, although potassium iodide and ammonium persulfate reduced activity. These properties suggest utility of pectinase and <TEX>${\beta}$</TEX>-glucosidase produced by B. subtilis B22 under blue LED-mediated fermentation