Plants regenerated from in vitro cultures carry chromosomal variations, especially in long-term culture. Reducingthe duration of plant tissue culture is one of the ways to reduce genetic and epigenetic changes. In this study, we reduced theduration of long-term culture and repeat subculture using small bulblets derived from bulb scales in two lily cultivars. Theadventitious bulblets derived from bulb-scale tissue were cultured on three different media containing Murashige and Skoog(MS) basal medium supplemented with 1 g/L Charcoal, MS medium containing 0.3 ㎎/L IAA and 0.4 ㎎/L BA hormonewith or without Charcoal, respectively. About seven weeks later, the number of newly propagated multiple shoots in the twomedia, A and B media, showed little differentiation. Compared to both media, the number of propagated multiple shootsincreased 5-fold in MS medium containing 0.3 ㎎/L IAA and 0.4 ㎎/L BA hormone without Charcoal (C medium). Thenumber of propagated multiple shoots ranged from 5 to 6 and 4 to 6 with an average of 5 in TropicalPink and GreenStarcultivars, respectively. The flow cytometric measurements indicated no variation in the ploidy level between control and invitro propagated plants.
ntroduction
Materials and Methods
Results and Discussion