[PURPOSE] The purpose of this study was to examine gender differences in the scores of FMS (Functional Movement Screen) in 20s adult. [METHODS] 83(50 male and 33 female) individuals in 20s adults who do not exercise regularly with no recent(<6 months) history of muscular skeletal injury were recruited for the study. The Functional Movement Screen consisted of 7 tests (DS; deep squat, IL; in-line lunge, HS; hurdle step, ASLR; active straight leg raise, SM; shoulder mobility, PU; trunk stability push up, RS; rotary stability. Mann-Whitney U tests were used to examine differences between male and female in the functional movement tests due to the rank-order nature of the data. [RESULTS] There was no statistically significant difference in total score between female(15.21±2.2) and male(15.38±2.4) while there was significant difference in SM, ASLR, PU between female and male. mean score of SM, ASLR, PU was 2.52±0.7, 2.32±0.7, 2.04±0.9 in male. mean score of SM, ASLR, PU was 2.87±0.3, 2.79±0.5, 1.27±0.7 in female. Female performed better on the SM, ASLR compared to male. The PU scores indicated that males were better than females in trunk stability. There was no significant difference in DS, IL, HS, and RS between females and males. [CONCLUSIONS] In conclusion, male and female of 20s adults may exhibit different qualities of functional movements. Therefore, strength and conditioning training program for sports injury prevention focused on improving functional movement in 20s adults should consider these gender differences.