This study was performed to investigate aflatoxin reduction resulting from the chemical treatments of corn. Aflatoxin was produced by Aspergillus parasiticus ATCC 15517 on a type of corn imported from the United States. The aflatoxin-produced corn(AC) was treated in several different chemicals for 2 hours at room temperature. The AC was also treated with 1, 2, 3, and 5% of sodium hypochlorite for 24 hours, or treated with 10% of sodium hypochlorite for 2, 24, 48, and 72 hours. The aflatoxin content in the samples was determined by high performance liquid chromatography. Out of the six chemicals tested as aflatoxin inhibitors, ammonium hydroxide 28% was found to be the most effective (99.11% reduction) (p<0.01) followed by sodium hydroxide 5%, sodium bicarbonate 5%, hydrochloric acid 0.1%, acetone 5%, and sodium hypochlorite 10%(p<0.01). A significant reduction of total aflatoxin in the AC treated with 1% solution of sodium hypochlorite was observed in 24 hours(p<0.01). The 10% solution of sodium hypochlorite showed a significant reduction of total aflatoxin in the AC in 2 hours(p<0.01). This study provides that atmospheric ammoniation of corn may be an effective method for feed. It could be emphasized, however, that approval for ammoniation of aflatoxin-contaminated corn has not been given by FDA, and it is likely that other safety studies using animals must be conducted before we can recommend the use of the process.
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