ICR mouse의 피부와 간에서 자외선 B파에 의한 Heme Oxygenase-1의 유도
Heme Oxygenase-1 (HO-1) Induction by UVB (290-320nm) Radiation in ICR Mice
- Choi, Wook-Hee Kim, Tae-Hwan Ahn, Ryoung-Me
- 1. 한국환경보건학회지
- 제34권 제1호
- 등재여부 : KCI우수등재
- 49 - 54 (6 pages)
The induction of heme oxygenase-1(HO-1) by UV radiation provides a protective defence against oxidative stress, and has been well demonstrated in skin irradiated with UVA, but not UVB. In this study, we show that the induction of cutaneous HO-l can be attributed to UVB radiation. The expression of HO-1 mRNA was assessed in vivo by reverse transcription-polymerase chain reaction (RT-PCR) analysis, and HO-1 enzyme activity was measured in microsomal preparation from irradiated mice. The mRNA level of HO-1 increases in liver and skin from 1d to 3d after UVB (3KJ/m2) exposure. The results of gene expression were same pattern of HO-1 enzyme activity in skin, but not in liver. HO-1 mRNA in liver resulted in a progressive increase to 4d after UVB exposure, but HO-1 activity in liver increased to 2d. This finding indicates that UVB radiation is an important inducer of HO-1 and increases in HO activity may protect tissue directly or indirectly from oxidative stress.