생물농약개발을 위한 활성미생물의 분리동정에 관한 연구

Isolation and Identification of Actives Microoraganisms forBiocide Development

An anti-fungal material produced by actinomycete was isolated from domestic soil. This actinomycete was identified as Streptomyces albogriseus by 16S rDNA sequence. YEME (yeast extract 4 g, malt extract 10 g, glucose 4 g, D.W 1l, pH 7.00.2) medium was used for production of anti-fungal materials. S. albogriseus was cultured in a shaking incubator for 2 weeks at 150 rpm and 25oC. An anti-fungal material produced by S. albogriseus was identified at 340 nm by uv/ vis- spectrometer and it showed powerful anti-fungal activity. This is the first report that secondary metabolite produced by S. albogriseus showed an activity against phytopathogenic fungi such as Collectrichum coccodes, Botrytis cinerea, Cladosporium cucumerinum, Didymella bryoniae.