헤드스페이스-가스크로마토그래피-질량분석법에 의한 체모 중포름알데하이드 측정법 연구

A gas chromatography/mass spectrometric method was developed for the determination of formaldehyde in hair. 0.3 mg of hair was placed in 10 ml headspace vial. 1.5 mM pentafluorophenylhydrazine solution (pH 2) in 0.03 M phosphoric acid and 20 l of 500 mg/l acetone-d6 as internal standard were added in vial and sealed tightly with cap. The solution was heated for 30 min at 90oC in heating block. The extraction, the derivatization and the evaporation were performed simultaneously. After heating of the solution, 0.5 ml of headspace was taken up and analyzed by gas chromatography-mass spectrometry (GC-MS). Low limit of detection (LOD) and Low limit of quantitation (LOQ) of formaldehyde were 0.5 and 1.5 ng/g, respectively. The method was used to analyze formaldehyde in rat hair after oral exposure. The developed method may be valuable to be used to analyze formaldehyde in human hair.