적색 양파에서 안토시아닌 함량 조절 양적형질유전자좌와 연관된 SNP 분자표지 개발

Development of Single Nucleotide Polymorphism Markers Linked to Quantitative Trait Loci Controlling Anthocyanin Content in Red Bulb Onion (Allium cepa L.)

The bulb onion (Allium cepa L.), one of the most important vegetables worldwide, contains various functional compounds suchas quercetin, allicin, and flavonoids. Red onions are rich in anthocyanins, a flavonoid that is a functional phytochemical with antioxidativeand anticancer activities. In the previous study, two quantitative trait loci (QTLs) (qAC4.1 and qAC4.2) controlling the anthocyanin contentwere identified on chromosome 4 in an F2 population derived from a cross between A. cepa ‘SP3B’ and ‘H6’. In this study, we developedsingle nucleotide polymorphism-based high-resolution melting (HRM) markers linked to QTLs qAC4.1 and qAC4.2. In addition, we constructeda new genetic linkage map of chromosome 4 using HRM markers and performed a QTL analysis. The QTL qAC4.1 was false, while qAC4.2was a major QTL. The QTL peak position, logarithm of the odds value, and phenotypic variance explained of qAC4.2 was 53.6 cM, 7.45, and 22.51%,respectively. Four HRM markers (AC4.2_65336.1_1123-HRM, AC4.2_53230.3_454-HRM, AC4.2_11999.1_756-HRM, and AC4.2_14596.1_345-HRM)within the QTL region of qAC4.2 were developed in this study. The average anthocyanin content of B (homozygous paternal) genotypes washigher than that of A (homozygous maternal) and H (heterozygous) genotypes for all markers. Consequently, these markers will be useful formarker-assisted selection to develop onion cultivars with high anthocyanin content.