CCl₄에 의한 간손상 모델 실험동물에 있어서 cyclohexane 투여가 혈청 glutathione S-transferase 활성에 미치는 영향

Effect of Cyclohexane Treatment on Serum Level of Glutathione S-Transferase Activity in Liver Damaged Rats

To evaluate the effect of cyclohexane(CH) treatment on the serum levels of glutathion S-transferase(GST) activity in liver damaged animals, damaged liver was induced with pretreatment of 50% CCl4 dissolved in olive oil (0.1 m1/100g body weight) intraperitoneally 17 times every other day. To CCl4-treated rats, CH (1.56 g/kg body weight, i.p) was injected once and then the animals were sacrificed at 4 hours after injection of CH. The CCl4-treated animals were identified as severe liver damage on the basis of liver functional findings, 1,e, increased serum levels of alanine aminotransferase(ALT), alkaline phosphate(ALP) and xanthine oxidase(XO) activities. On the other hand, CCl4-treated animals injected with CH once(CCl4-pretreated animals) showed more decreased serum levels of ALT and XO, and more increased those of ALP rather than CCl4-treated animals. In case of comparing the GST with ALT activity in liver, both CCl4-treated and pretreated animals showed similar changing pattern of enzyme actvity. Especially CCl4-pretreated animals showed significantly increased serum level of GST actvity compared with the CCl4-treated those, whereas those of ALT showed reversed tendency. In aspects of GST enzyme kinetics, CCl4-pretreated animals showed higher Vmax of liver GST enzyme than CCl4-treated animals. In conclusion, injection of CH to the liver damaged rats led to enhanced liver damage and more increased activity of serum GST which may be chiefly caused by the enzyme induction.