We examine the metabolites(DCB and acetyl DCB) extracted from exfoliated urothelial cells of 33 workers who employed DCB-handling industries. The characteristics of workers submitted urine, whose age, working years and smoking persons were 41.9±11.1, 8.7±5.5 and 25(32.0%), respectively. DNA adduct was isolated from the exfoliated urothelial cells by applying 32p-postlabeling procedure. Metabolites(DCB and acetyl DCB) were extracted from DNA adducts by hydrolyzing and N-glycosylase. Concentrations of DCB and acetyl DCB were 28.6±5.25 ng/g DNA, and 17.0±3.73 ng/g DNA, respectively. The regression between DCB level and exposure years of workers is y = 1.668 + 2.588x(p = 0.005, r2= 0.394). The regression between acetyl DCB level and exposure years of workers is y = 8.071 + 1.325x(p = 0.076, r2= 0.222). Smoking workers are significantly higher than non-smoking workers on DCB and acetyl DCB level(p = 0.065 and 0.021, respectively). DCB level was 33.9±7.14 ng/g DNA on smokers, and 23.1±9.97 ng/g DNA on non-smokers. Acetyl DCB was 25.1±5.27 ng/g DNA on smokers, and 8.92±7.22 ng/g DNA on non-smokers.
Materials and Method