랫드를 이용한 phosalone의 대사산물 측정

Determination of Urinary Metabolites of Phosalone in Rats

This study was performed to determine urinary metabolites of phosalone in rats . Phosalone, one of the organophosphorus pesticides, was orally administrated to rats. Urine samples were collected for 24hr in metabolic cages. Four metabolites, DEP, DETP, DEDTP, chlorobenzoxazone, were detected in the rat urine. No parent compound was detected. In GC/MS, metabolites are identified on the basis of their mass spectra. The molecular ion of DEP, DETP, DEDTP and chlorobenzoxazone were m/z=181, mlz=226 , mlz=242, and m/z=169, respectively. Nearly all of the diethyl metabolites were excreted within 48hr after administration of phosalone. The method of liquid-liquid extraction without purification and butylation of dialkyl phosphates using tetrabutylammonium hydroxide reagent was achieved in the GCFPD and GC/MS injection block at 280°C. Average recoveries(n=3) and coefficients variation(% CV) of DEP and DEDTP from spiked blank urine samples with 5% ethanol in ethyl acetate were 78 .2%( 46%) and 98.0%(22%), respectively. The limits of detection for these compounds with 2 μl injections was 0.5 ppm for DEP and 0 .lppm for DEDTP by GC-FPD.