임신랫드에 투여한 Butyl Benzyl Phthalate가 차산자에 미치는 영향
The Effects of Butyl Benzyl Phthalate on Pregnant Rats and their Fetuses
Butyl benzyl phthalate(BBP) is a member of phthalic acid esters(PAEs) and is used extensively as a plasticizer in floor tiles, adhesives, synthetic leathers and hairspray. BBP is reported to be developmentally toxic, but it was to be nonregulated chemicals by Ministry of Environment in Korea. This study was investigated on the effect of BBP treatment on during gestational period of the dams and their fetuses. Sprague-Dawley rats were given BBP by oral administration at 0, 10, 100, 1000 mg/kg on days 1 to 21 of gestational period. One half of them was sacrificed at 20 days of gestation, and the rest was sacrificed at post parturition 21 days. Approximately one half of live fetuses in the gestational 20 days were randomly selected, fixed in 95% ethanol, stained alizarin red S and examined for skeletal malformations. At maternal findings, there were some significant changes in organ weight compared with control groups, especially in absolute liver weight and absolute kidneys weights by BBP but it was not dose dependent. As the BBP dose was increased; the uterus and ovary weight of treated dams were decreased in absolute and relative organ weight, except of 1000 mg/kg group. The post-implantation loss per litter was significantly increased at 1000 mg/kg treated group. As the BBP dose increased, the more dams showed estrus cycle changes such as estrus, proestrus earlier thaμ the control. In BBP 1000 mg/kg treated group, almost all pregnant rats had miscarried. At fetus findings, the body weights were decreased by administration of BBP. In female(F1) rats, there were significant changes in absolute and relative liver weight in BBP 10 mg/kg treated group. In genital organs, there were significant changes in absolute uterus weight in BBP 10 mg/kg treated group and there were inclined to increase of uterus weight in BBP 100 mg/kg treated group. In contrast, more high-treated group was inclined to decrease of ovary weight. In male(Fl) rats, there were significant changes in absolute and relative prostate weight in BBP 10 and 100 mg/kg treated group. Also, there were significant changes in absolute and relative testis weight in BBP 10 and 100 mg/kg treated group. The incidences of external malformations in fetuses were not examined. But skeletal variations were observed in all group. From these results, it could be concluded that the effects of pregngnt rats and their fetuses(F1) treated with BBP during day 1 to 20 of gestational period were estrogenic.
II. 실험 재료 및 방법
III. 결과 및 고찰