GC-MS-SIM과 ³²P-postlabeling방법에 의한 염료와 안료를 제조하는 사업장 근로자들의 방광암 조기발견을 위한 Biomarker개발에 관한 연구

A Study on Biomarker for Bladder Cancer Screening Workers Employed for Dyes and Pigments Manufacturing Industries by GC/MS-SIM and ³²P-postlabeling Method

To develop the biomarker of non-invasive biomonitoring among workers who exposed benzidine-based dyes in its manufacturing and treating industries, we examined and evaluated the specific carcinogen-DNA adducts in exfoliated urothelial cells of 33 exposed workers. Samples were collected from urine of workers, and then isolated DNA from them. DNA adducts were analyzed using ³²P-postlabeling, along with Nuclease P₁. One adducts was predominated in those samples with 6 adducts present. It was formed in all exposed workers, but the others not. Its relative adducts level(RAL) was 73.2 × 10&#8311;, which was statistically significantly higher than any other adducts levels(p<0.05) . Analyzing regression between its RAL and employment period of workers, simple linear regression was positively increased with slop of 6.922(p<0.05, r²=0.548). And also, DNA adducts were analyzed using GC/MS-SIM, along with n-butanol extraction and trifluoroacetyl anhydride(TFA) derivatization. Standard material was synthetic N( deoxyguanosin-8-yl)-N -acetylbenzidine. The characteristics of sample and standard were observed on the same sites, such as M&#8314;(m/z 376) and M&#8314;(m/z 279) for benzidine-di-TFA and mono-TFA. And then, they were confirmed by analyzing with secondary ion mass(SIM). This study showed that benzidine-based dyes was formed one DNA adduct into significantly high levels in bladder of all exposed workers, its chemical formula was N-(deoxyguanosin-8-yl)-N acetylbenzidine. So we can do monitoring the workers with exfoliated urothelial cells of them by analyzing above DNA adduct as biomarker for preventing bladder cancer causing the exposure of the benzidine-based dyes.