1, 2, 4-Benzenetriol에 의해 유도된 HL-60 세포의 DNA 손상

Detection of 1, 2, 4-Benzenetriol Induced DNA Damage by Single Cell Gel Electrophoresis

One of the carcinogenic mechanisms of benzene is believed to be the oxidative damage caused by its metabolites. The triphenolic metabolite of benzene, 1,2,4-benzenetriol(BT) is readily oxidized to its corresponding quinone via semiquinone radical. During this process reactive oxygen species are formed that may damage cellular DNA and induce genotoxic effects. A comparative study was carried out to examine the effect of BT on DNA damage by single cell gel electrophoresis assay(SCGE), chromosome aberration and lipid peroxidation production analysis. HL-60 cells were exposed to BT for 3 hours and the extent of DNA migration was measured by a single cell gell electrophoresis under alkaline conditions. BT induced DNA damage in a dose-dependent manner at concentrations between 10 μM and 100 μM and most of the cell could repair DNA damage inunediately following exposure to the chemical. BT also induced chromosome aberration and most frequent type of chromosome aberration is chromatid breaks. BT induced lipid peroxidation production. From above results, it is suggested that BT can induce DNA damage and chromosome aberration through production of reactive oxygen species(ROS).