독소원성대장균이 생산하는 장독소의 검색을 위한 RPLA 법과 PCR기법의 감도비교
Comparison of Sensitivity of Detection for Enterotoxigenic Escherichia coli Enterotoxin by use of the Reversed Passive Latex Agglutination and the Polymeras Chain Reaction
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초록
Detection for heat-labile enterotoxin(LT) of enterotoxigenic Escherichia coli(ETEC) by use of the reversed passive latex agglutination(RPLA) was positive reaction from 2-fold(50 ng/ml) to 64-fold dilutions in EC81 and EC83. The polymerase chain reaction(PCR) which was using LT gene-specific primers of ETEC with a detection limit equivalent from 1 ng/㎕ to 1 pg of a DNA fragment of 417-bp in EC81 and EC83. The PCR provided a rapid and more sensitivity tool for the detecting LT of ETEC compare to the RPLA.
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II. 실험재료 및 방법
III. 결과 및 고찰
IV. 결론
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