The biosynthesis of galactolipid and the composition of fatty acid were analyzed in plasma membrane isolated from C. ellipsoidea treated with antiseptics (potassium sorbate; PS, sodium benzoate; SB, calcium propionate; CP) and surfactantsOinear alkylbenzene sulfonate; LAS, α-olefin sulfonate; AOS, sodium lauryl ether sulfate; SLES) during the culture. The contents of MGDG, DGDG and total lipid in the antiseptics treatments and the surfactants treatments were lower to compared with the control. The major fatty acid utilized for biosynthesis of MGDG were linolenic acid(ave. 14.40%), palmitoleic acid(ave. 12.97%) in control. Otherwise, the major fatty acids in P.S treatment were u - tilized for oleic acid(ave. 15.69%), linolenic acid(ave. 11.16%), and palmitoleic acid(ave. 12.01%), oleic acid(ave. 11.42%) in S.B treatment, and oleic acid(ave. 10.22%), linoleic acid(ave. 16.71%) in C. P treatment. It was showed that the major fatty acids in plasma membrane DGDG were oleic acid (ave. 16.38%), linolenic acid(ave. 11.16%) in control, oleic acid(ave. 15.69%), linolenic acid(ave. 11. 16%) in P.S treatment, and palmitoleic acid(ave. 11.16%), oleic acid(ave. 11.42%) in S.B treatment, and oleic acid(ave. 10.22%), linoleic acid(ave. 16.71%) in C.P treatment. The major fatty acids in plasma membrane MGDG of C. ellipsoidea treated with various surfactants, oleic acid(ave. 11.11 %), linolenic acid(ave. 9.21%) in LAS treatment, linolenic acid(ave. 16.22%), oleic acid(ave. 14.71%) in AOS treatment, linoleic acid(ave. 13.01%), linolenic acid(ave. 11.63%) in SLES treatment. Otherwise in DGDG biosynthesis, heptadecanoic acid(ave. 14.50%), stearic acid(ave. 11.84%) in LAS treatment, palmitoleic acid(ave. 16.90%), oleic acid(ave. 11.10%) in AOS treatment, linoleic acid(ave. 14.78%), arachidic acid(ave. 8.01%) in SLES treatment.
I. 서론
II. 재료및방법
III. 결과
IV. 고찰
V. 결론