납이 HL 60 세포 내 Myo-inositol 수송 체계에 미치는 영향

Effect of Lead on the Myo-inositol Transport System in HL 60 Cell Line

Lead is the most ubiquitous toxic metal and released to the environment from natural and man-made sources. Presence of lead in the environment has led to an increasing awareness and concern of deterimental effect on ecosystem and human health. But there are several limits for toxicity assessment and protection human from toxicity of lead because the toxic effects of lead are detectable in practice only at high exposure concentraion. Therfore, it is very important to search sensitive biomakers at low exposure concentration which could not be observed any practical symptoms such as anemia, encephalopathy, and neuropathy in human. In the present study, to investigate myo-inositol concentration in cell could be a biomaker for lead cytotoxicity, the toxic effects of lead on myo-inositol uptake system were studied in the HL 60 cell line from three groups : normal control, lOppm-lead treated group, 80 ppm-lead treated group. Lead significantly reduced specific myo-inositol uptake in lead-treated groups, but did not affected nonspecific uptake. The magnitude of reduction in myo-inositol uptake was directly propotional to exposure dose. But myo-inositol uptakes in lead treated groups were significantly normalized by addition sodium ion to culture medium. These results suggest that myo-inositol concentration in the cell could be a useful biomaker in toxicity test with cell line and the toxic mechanism of lead on HL 60 cell might be a reduction in myo-inositol uptake, which induced by the perturbation of the sodiumion metabolism.