몰로키아(Corchorus olitirius L.) 추출물의 생리활성에 대한 연구

Study on the Physiological Activity of Corchorus olitirius L.

In this study, the effects of the physiological activity of the Corchorus olitirius L. were verified and the applicability as a cosmetic material was reviewed. The total polyphenol content was expressed as 396.44±0.42 mg/100g dry in the water extract of Corchorus olitirius L. (COWE) and the content was expressed as 458.13±0.42 mg/100g dry in the 70% ethanol extract of Corchorus olitirius L. (COEE). The antioxidant effects were measured through an experiment evaluating the electron-donating ability and ABTS radical scavenging activities. In order to verify the whitening effects, tyrosinase inhibitory activity was measured and results showed an effect of 11.82% (COWE) and 48.21% (COEE) at 1,000 μg/mL, respectively. An MTT assay was conducted in RAW 264.7 and B16F10 cells to assess the cell survival rate; it was found that no toxicity was apparent at 100 μg/mL. Active suppression of nitric oxide (NO), an inflammation inducer, was measured to verify the anti-inflammatory efficacy. In the NO assay, experiments were conducted using a concentration of 100 μg/mL which led to a 95% survival rate. The NO assay was measured using Griess reagent and compared to the lipopolysaccharide treatment group and non-treatment group. The inhibition of cellular melanin synthesis was measured to determine the whitening effects. At 100 μg/mL, the inhibition activities of COWE and COEE were 15.69% and 22.67%, respectively. It has been confirmed that COWE and COEE are effective antioxidants, have whitening effects, and are anti-inflammatory agents. In particular, the use of COWE and COEE as natural materials for cosmetics was confirmed to have excellent effects.