
콩 종자의 Raffinose 및 Stachyose 함량에 대한 유전자형과 환경의 영향
Genotype and Environment Influence on Raffinose and Stachyose Content of Soybean Seed
- 성미경(Mi Kyung Sung) 한성진(Sung Jin Han) 서형진(Hyung Jin Seo) 최상우(Sang Woo Choi) 남상해(Sang Hae Nam) p정종일(Jong Il Chung)
- 한국작물학회
- 한국작물학회지
- 제59권 제3호
- 등재여부 : KCI등재
- 2014.09
- 319 - 324 (6 pages)
Soybean (Glycine max (L.) Merr.) is an important crop for protein, oil, carbohydrates, isoflavones, and many other nutrients to humans and animals. But, antinutritional factors in the raw mature soybean are exist. Raffinose and stachyose are main antinutritional factors in soybean seed. Both raffinose and stachyose are carbohydrates, belonging to the raffinose family of oligosaccharides (RFOs). RFOs are not readily digested in humans and cause flatulence or diarrhea. The objective of this research is to obtain the information on raffinose and stachyose content according to genotype and environment. A total of twenty two soybean genotypes (11 cultivars, 3 germplasms and 8 breeding lines) were selected. Each genotype was grown in the field for two years with two replications and harvested in bulk at natural maturity for two years. Content of raffinose and stachyose was detected by HPLC. The raffinose content (g/kg) of 22 genotypes was 2.68±0.21 - 5.87±2.43 in year 1 and was 3.24±0.37 - 9.05±0.16 in year 2. The stachyose content (g/kg) was 4.23±0.98 - 27.68±9.90 at year 1 and was 5.11±1.09 - 25.32±0.35 in year 2. Genotype and environment have highly significant effects on raffinose and stachyose content. Three genotypes (Da-7, 116-13, and RS-78) have low stachyose content at 5% significant level in two years. A positive correlation (R2=0.1985*) between raffinose and stachyose was observed in year 2. These informations are valuable in soybean genetics and breeding program related with raffinose and stachyose content.
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