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KCI등재 학술저널

옥수수 종실 및 속대의 Phytosterol 동정과 함량 변이

Identification and Quantification of Phytosterols in Maize Kernel and Cob

DOI : 10.7740/kjcs.2018.63.2.131
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Unsaponifiables in the kernel and the cob of 7 maize varieties were analyzed by using thin-layer chromatography (TLC) and gas chromatography (GC) for the identification of phytosterols and their concentrations. The unsaponifiables of the kernel were clearly separated into band I (campesterol, stigmasterol, and β-sitosterol), band Ⅱ (Δ5-avenasterol), band Ⅲ (Δ7- stigmastenol), and band Ⅳ (Δ7-avenasterol). In the cob, on the other hand, three or more bands were separated in addition to bands. The GC analysis of unsaponifiables showed good separation of campesterol, stigmasterol and β-sitosterol, but the mixture of Δ7- avenasterol (retention time[RT] 22.846), Δ7-stigmastenol (RT 22.852), and Δ5-avenasterol (RT 22.862) showed poor separation. Phytosterol content of the maize kernel was 635.9 mg/100 g, and that of the cob was 273.0 mg/100 g, respectively. The phytosterol content of the kernel was 2.4-fold higher than that of the cob. The phytosterol content of the kernel was higher in the order β- sitosterol 80.05% > campesterol 10.5% > stigmasterol 9.46%, but that of the cob was higher in the order β-sitosterol 59.43% > stigmasterol 31.72% > campesterol 10.98%. Based on these results, it appears that the phytosterols of the maize kernel are synthesized in the maize cob and are transferred to the kernel, because the precursors (Δ7-avenasterol, Δ7-stigmastenol, and Δ5- avenasterol) of major phytosterols were detected in maize cobs.

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