Verification of Genetic Purity of Soybean F₁ Hybrid Using Molecular Markers

Soybeans have an extremely low success rate of artificial crossings, which requires a lot of effort from breeders. Moreover, the morphological characteristics of the F1 hybrid seeds are difficult to assess visually if similar to the phenotypes of the paternal and maternal parents. Therefore, an accurate genetic purity test of F1 hybrid plants is essential step for breeding purposes. We used polymerase chain reaction (PCR)-based molecular markers as a tool for hybrid seed purity determination to breed Soybean (Glycine max L.) varieties for natto (unripe Cheonggukjang).