근적외선분광분석에 의한 동아시아 지역 재래종 벼 유전자원의 아밀로스 및 단백질 함량 변이분석

Statistical Analysis of Amylose and Protein Content in Landrace Rice Germplasm Collected from East Asian Countries Based on Near-Infrared Reflectance Spectroscopy (NIRS)

A statistical analysis of 4,380 non-glutinous landrace rice germplasm collected from four East Asian countries namely South Korea (1,032), North Korea (994), Japan (800), and China (528) was conducted using normal distribution, variability index value (VIV), analysis of variation (ANOVA), and Duncan’s multiple range test (DMRT) based on a data obtained from Near-Infrared Reflectance Spectroscopy (NIRS) analysis. In normal distribution, the average protein content was 8.2%, and the non-glutinous rice amylose, ranging over 10%, was found to be 22.0%. Protein content in most gremplasm was between 5.4 and 10.9%, and amylose content was between 15.0 and 28.9%. The VIV was 0.50 for protein, and 0.81 for non-glutinous rice amylose content. The average amylose content was 23.34% in Chinese, 21.55% in South Korean, 21.45% in Japanese, and 20.48% in North Korean resources, while the average protein content was found to be 9.02% in Chinese, 8.06% in Japanese, 8.04% in North Korean, and 7.99% in South Korean resources. ANOVA of amylose and protein content showed significant differences at p=0.01. The F-test value for amylose content was 94.92, and for protein content was 81.82 compared to the critical value of 3.79. DMRT of amylose and protein content revealed significant differences (p<0.01). Among the various germplasm obtained from different countries, that from North Korean had the lowest level of amylose content, whereas that from South Korea had the lowest level of protein content than all other resources. Chinese resources had the highest level of amylose and protein content. It is recommended to use these results in breeding fields.