Rare Cold Inducible 2 Proteins are Internalized from Plasma Membrane to Endomembranes under NaCl Stress in Camelina sativa L.

Rare Cold Inducible 2 (RCI2A to H) proteins are small size hydrophobic protein known to be localized at plasma membrane (PM). Expression of RCI2s are significantly induced by abiotic stresses such as cold, drought, and saline stress. Accumulation or over-expression of RCI2s contributes abiotic stresses tolerance but a cellular function remains unclear. In previous, we reported novel protein interaction between CsRCI2E/F and aquaporin CsPIP2;1 which is reduces water transport activity. Endocytic trafficking of aquaporin PIP2;1 under NaCl or osmotic stress have been reported in several species but not in CsRCI2s. In this study, we observed vesicle trafficking using fluorescence protein fusion at N-terminal of CsRCI2s. In addition, changes of protein accumulation in different membrane fraction such as PM and tonoplast. From these results we would like to describe novel function of CsRCI2s internalization under NaCl stress.