스피페론에 의한 멜라닌 생성에 미치는 칼슘-활성화 염소통로의 역할

Role of Ca2+-activated Cl−Channels in the Spiperone-mediated Melanogenesis

The molecular mechanism underlying the stimulation of melanin synthesis by the antipsychotic drug spiperone (SP) was investigated in B16 melanoma cells. SP increased the melanin content and intracellular Ca2+ ion concentration in a dose-dependent manner. Treatment with bis-(o-aminophenoxy)-ethane-N,N,N ,N -tetraacetic acid/acetoxymethyl ester, an intracellular Ca2+ chelator, significantly inhibited SP-induced melanin synthesis. SP induced considerable Cl− efflux, which was inhibited by niflumic acid and flufenamic acid, known Ca2+-activated Cl− channel (CaCC) blockers. Furthermore, these CaCC blockers significantly inhibited SP-induced melanin synthesis. Thus, these results suggest that CaCCs may play an important role in SP-induced melanin synthesis in melanoma cells.